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YY1 Polyclonal Antibody

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse
Overview
Catalog # bs-53085R
Product Name YY1 Polyclonal Antibody
Applications WB, ELISA
Reactivity Human, Mouse
Specifications
Conjugation Unconjugated
Host Rabbit
Source Polyclonal antibody raised in rabbit against human YY1 (YY1 transcription factor) using two KLH-conjugated synthetic peptides containing a sequence from the central region of the protein
Clonality Polyclonal
Isotype Not Determined
Concentration Not Determined
Purification Whole antiserum from rabbit containing 0.05% azide
Storage Buffer Serum with 0.05% sodium azide.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 7528
Swiss Prot P25490
Synonyms INO80S, NF-E1, DELTA, UCRBP, YIN-YANG-1
Background Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions).
Application Dilution
WB 1:300-5000
ELISA 1:500-1000