DAXX (Ser517) Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-DAXX (Ser517) Polyclonal Antibody (bs-5292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-DAXX (Ser517) Polyclonal Antibody (bs-5292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Lane 1: Human K562 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human PANC-1 cell lysates probed with \r\nphospho-DAXX Polyclonal Antibody, Unconjugated (bs-5292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human K562 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human PANC-1 cell lysates probed with \r\nphospho-DAXX Polyclonal Antibody, Unconjugated (bs-5292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-5292R
Product Name	DAXX (Ser517) Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human DAXX around the phosphorylation site of Ser517
Modification Site	Ser517
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1616
Subcellular location	Cytoplasm, Nucleus
Synonyms	BING 2; BING2; DAP 6; DAP6; Death associated protein 6; Death domain associated protein 6; EAP 1; EAP1; ETS1 associated protein 1; Fas death domain associated protein; hDaxx; MGC126245; MGC126246; DAXX_HUMAN.
Background	Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD) containing adapter molecules and members of the ICE/CED3 protease family. A novel DD containing molecule was recently cloned from mouse, human and monkey and designated Daxx. Daxx is a death domain containing important intermediate in the Fas mediated apoptosis. Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N terminal kinase (JNK) pathway. It is widely expressed in fetal and adult human and mouse tissue, indicating its important function in Fas signaling pathways.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200