| Overview |
| bs-5218R-APC |
| Wee1(Ser123) Polyclonal Antibody, APC Conjugated |
| WB, IF(IHC-P), IF(IHC-F), IF(ICC) |
| This phosphorylation site is homologous to Ser123 in Mouse, Rat, and Bovine. The peptide used to produce this antibody has high homology with a sequence occurring in MIER3, and may react with this protein in Mouse and Human when phosphorylated at the site of Ser10. |
| Mouse, Others |
| Human, Rat, Dog, Cow, Pig, Rabbit |
| Specifications |
| APC |
| Rabbit |
| KLH conjugated synthetic phosphopeptide derived from human Wee1 around the phosphorylation site of Ser123 [SS(p-S)PV] |
| Ser123 |
| Polyclonal |
| IgG |
| 1ug/ul |
| Purified by Protein A. |
| Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target |
| 7465 |
| P30291 |
| Nucleus |
| WEE1A; WEE1hu; Wee1-like protein kinase; Wee1A kinase; WEE1 |
| Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. |
| Application Dilution |
| WB |
1:300-5000 |
| IF(IHC-P) |
1:50-200 |
| IF(IHC-F) |
1:50-200 |
| IF(ICC) |
1:50-200 |
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