LASS5 Polyclonal Antibody

Mouse Eye lysates probed with LASS5 Polyclonal Antibody, Unconjugated (bs-5082R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS5 Polyclonal Antibody, Unconjugated (bs-5082R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LASS5 Polyclonal Antibody, Unconjugated (bs-5082R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-5082R
Product Name	LASS5 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	Possible cross-reactivity with CERS6/LASS6 (85% homology with the immunogen sequence)
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LASS5
Immunogen Range	101-200/392
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	55890
Swiss Prot	Q8N5B7
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	LAG1 homolog ceramide synthase 5; LAG1 longevity assurance homolog 5; MGC45411; Trh4; CerS5; FLJ25304; CERS5_HUMAN.
Background	Lass5, or LAG1 longevity assurance homolog 5, is thought to be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donnor ceramide) in a fumonisin B1-independent manner.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200