MRC1 Polyclonal Antibody

$HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody\uff08bs-4727R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody\uff08bs-4727R\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Polyclonal Antibody(bs-4727R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse
Rat
Dog

Predicted Reactivity

Cow
Sheep
Pig
Horse
Chicken
Guinea Pig

Overview
Catalog #	bs-4727r-100ul
Product Name	MRC1 Polyclonal Antibody
Applications	WB, ELISA, FCM
Reactivity	Human, Mouse, Rat, Dog
Predicted Reactivity	Cow, Sheep, Pig, Horse, Chicken, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MRC1
Immunogen Range	201-300/1456
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4360
Swiss Prot	P22897
Subcellular location	Cell membrane
Synonyms	MMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1; Macrophage mannose receptor 1; C-type lectin domain family 13 member D; C-type lectin domain family 13 member D-like; Macrophage mannose receptor 1-like protein 1; MRC1
Background	Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100