DSN1 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (DSN1) Polyclonal Antibody, Unconjugated (bs-42142R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Dog
Cow
Sheep
Horse
Rabbit

Overview
Catalog #	bs-42142R
Product Name	DSN1 Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Dog, Cow, Sheep, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human DSN1 protein
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79980
Swiss Prot	Q9H410
Subcellular location	Nucleus
Synonyms	C20orf172; dJ469A13.2; DSN1; DSN1, MIND kinetochore complex component, homolog; DSN1, MIND kinetochore complex component, homolog (S. cerevisiae); DSN1_HUMAN; FLJ13346; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore-associated protein DSN1 homolog; MGC32987; MIS13.
Application Dilution
ELISA	=1:5000-10000
IHC-P	IHC-P=1:100-500