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CD74 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD74) Polyclonal Antibody, Unconjugated (bs-41374R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD74) Polyclonal Antibody, Unconjugated (bs-41374R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • ELISA
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
Overview
Catalog # bs-41374r-100ul
Product Name CD74 Polyclonal Antibody
Applications ELISA, IHC-P, IHC-F, IF
Reactivity Mouse
Predicted Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source Recombinant mouse CD74 protein
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification affinity purified by Protein A
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 972
Swiss Prot P04233
Subcellular location Cell membrane
Synonyms CD 74; CD74 antigen; CD74 molecule; CLIP; DHLAG; Gamma chain of class II antigens; HG2A_ ; HLA class II histocompatibility antigen gamma chain; HLA DR antigens associated invariant chain; HLA DR gamma; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLADG; HLADR antigens associated invariant chain; Ia antigen associated invariant chain; Ia antigen-associated invariant chain; Ia associated invariant chain; Ia gamma; Ii; Invariant polypeptide of major histocompatibility complex class II antigen associated; la-gamma; Major histocompatibility complex class II invariant chain; MHC HLA DR gamma chain; MHC HLA-DR gamma chain; p33; p35; Protein 41.
Application Dilution
ELISA =1:5000-10000
IHC-P IHC-P=1:400-800
IHC-F IHC-F=1:400-800
IF IF=1:100-500

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