Histone H3 (acetyl K9) Antibody

Lane 1:Huh7 lysates Lane 2: A549 lysates probed with Rabbit Anti-Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Lane 1: NIH\/3T3 cell lysates; Lane 2:Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: U2os cell lysates probed with Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: NIH\/3T3 cell lysates; Lane 2:Hela cell lysates; Lane 3: MCF-7 cell lysates; Lane 4: U2os cell lysates probed with Histone H3 (acetyl K9) Polyclonal Antibody, Unconjugated (bs-3776R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Pig
Rabbit
Drosophila

Overview
Catalog #	bs-3776R
Product Name	Histone H3 (acetyl K9) Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	This acetylation site is homologous across the species listed.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Pig, Rabbit, Drosophila
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic acetylpeptide derived from human Histone H3 around acetylation site of Lys9
Modification Site	acetyl K9
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3020
Swiss Prot	P84243
Subcellular location	Nucleus
Synonyms	H3F3; H3.3A; Histone H3.3; H3F3A; PP781
Background	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200