P70 S6 Kinase beta 2 (Ser370) Antibody

$Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-P70 S6 Kinase beta 2 (Ser370) Antibody(bs-3701R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with phospho-P70 S6 Kinase beta 2 (Ser370) Antibody(bs-3701R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

WB
ELISA

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-3701R
Product Name	P70 S6 Kinase beta 2 (Ser370) Antibody
Applications	WB, ELISA
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human P70 S6 Kinase beta 2 around the phosphorylation site of Ser370
Modification Site	Ser370
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Nucleus
Synonyms	RPS6KB2 phospho S370; RPS6KB2 phospho Ser370; p-RPS6KB2 Ser370; 70 kDa ribosomal protein S6 kinase 2; p70 beta; p70 ribosomal S6 kinase beta; p70 S6 kinase beta; p70 S6KB; p70 S6Kbeta; p70S6K beta; p70-beta; p70-S6KB; p70S6Kb; Ribosomal protein S6 kinase 70kDa, polypeptide 2; Ribosomal protein S6 kinase beta 2; Ribosomal protein S6 kinase beta-2; RPS6KB2; S6 kinase related kinase; S6 kinase-related kinase; S6K beta 2; S6K beta; S6K-beta; S6K2; Serine/threonine protein kinase 14 beta; Serine/threonine-protein kinase 14 beta; SRK; STK14B; KS6B2_HUMAN; S6KB2.
Background	Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000