PFK2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat stomach labeled with Anti-PFK2 Polyclonal Antibody, Unconjugated (bs-3528R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human Jurkat cell lysates probed with PFKFB3\/PFK2 Polyclonal Antibody, Unconjugated (bs-3528R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HepG2 cell lysates; Lane 2: Human HeLa cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human Jurkat cell lysates probed with PFKFB3\/PFK2 Polyclonal Antibody, Unconjugated (bs-3528R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-3528R
Product Name	PFK2 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PFK2
Immunogen Range	401-520/520
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5209
Swiss Prot	Q16875
Subcellular location	Cytoplasm, Nucleus
Synonyms	PFK2; IPFK2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; 6PF-2-K/Fru-2,6-P2ase 3; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; Renal carcinoma antigen NY-REN-56; iPFK-2; PFKFB3
Background	Synthesis and degradation of fructose 2,6-bisphosphate.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200