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PKC alpha/beta 2 (Thr638/641) Antibody, APC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat
  • Dog
  • Others

Predicted Reactivity

  • Human
  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-3333R-APC
Product Name PKC alpha/beta 2 (Thr638/641) Antibody, APC Conjugated
Applications WB, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse, Rat, Dog, Others
Predicted Reactivity Human, Cow, Horse, Chicken
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human PKC alpha/beta II around the phosphorylation site of Thr638/641
Modification Site Thr638/641
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5578
Swiss Prot P17252
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms AAG6; PKCA; PRKACA; PKC-alpha; Protein kinase C alpha type; PKC-A; PRKCA
Background Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200