FoxO3a (Ser318 + Ser321) Antibody

$Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Phospho-FoxO3a (Ser318\/321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Phospho-FoxO3a (Ser318\/321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO3a (Ser318 + Ser321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with FoxO3a (Ser318 + Ser321) Polyclonal Antibody, Unconjugated (bs-3138R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-3138R
Product Name	FoxO3a (Ser318 + Ser321) Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	These phosphorylation sites are homologous to Ser317 + Ser320 in Mouse and Rat. Due to the highly conserved nature of this sequence, there is a chance that this antibody will react with FoxO1a when phosphorylated at the the sites of Ser322 and Ser325 in Human, Ser319 and Ser322 in Mouse, and Ser316 and Ser319 in Rat.
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser318/321
Modification Site	Ser318 + Ser321
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2309
Swiss Prot	O43524
Subcellular location	Cytoplasm, Nucleus
Synonyms	FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3
Background	Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200