Cyclin B1 (Ser133) Polyclonal Antibody

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser133)) polyclonal Antibody, Unconjugated (bs-3123R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Phospho-Cyclin B1 (Ser133)) polyclonal Antibody, Unconjugated (bs-3123R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
FCM

Predicted Reactivity

Human
Mouse
Rat
Pig

Overview
Catalog #	bs-3123R
Product Name	Cyclin B1 (Ser133) Polyclonal Antibody
Applications	WB, ELISA, FCM
Predicted Reactivity	Human, Mouse, Rat, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser133
Modification Site	Ser133
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	891
Subcellular location	Cytoplasm, Nucleus
Synonyms	Cyclin B1 phospho S133; Cyclin B1 Ser133; p-Cyclin B1 S133; p-Cyclin B1 Ser133; p-Cyclin B1 phospho S133; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1.
Background	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100