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ALK (Y1278 + Y1282 + Y1283) Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-3021R-Biotin
Product Name ALK (Y1278 + Y1282 + Y1283) Antibody, Biotin Conjugated
Applications ELISA, IHC-P, IHC-F
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human ALK around the phosphorylation site of Tyr1278/1282/1283
Modification Site Tyr1278 + Tyr1282 + Tyr1283
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 238
Swiss Prot Q9UM73
Subcellular location Cell membrane
Synonyms CD246; NBLST3; ALK tyrosine kinase receptor; Anaplastic lymphoma kinase; ALK
Background Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500