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eIF4EBP1 (Thr37 + Thr46) Antibody, Cy5 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-3019R-Cy5
Product Name eIF4EBP1 (Thr37 + Thr46) Antibody, Cy5 Conjugated
Applications WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46
Modification Site Thr37 + Thr46
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1978
Swiss Prot Q13541
Subcellular location Cytoplasm, Nucleus
Synonyms BP-1; 4EBP1; 4E-BP1; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; EIF4EBP1
Background Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200