BMI1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti BMI1\/PCGF4 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti BMI1\/PCGF4 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Human MCF-7 cell lysates probed with PCGF4\/BMI1 Polyclonal Antibody, Unconjugated (bs-2999R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature , permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PCGF4\/BMI1 Polyclonal Antibody(bs-2999R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

NIH\/3T3 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PCGF4\/BMI1 Polyclonal Antibody(bs-2999R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).