MLK3 Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse heart labeled with Anti-MLK3\/RHOE Polyclonal Antibody, Unconjugated (bs-2992R) at 1:200 followed by conjugation to the secondary antibody$

Formalin-fixed and paraffin embedded mouse heart labeled with Anti-MLK3\/RHOE Polyclonal Antibody, Unconjugated (bs-2992R) at 1:200 followed by conjugation to the secondary antibody

$Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-MLK3\/RHOE Polyclonal Antibody, Unconjugated (bs-2992R) at 1:200 followed by conjugation to the secondary antibody$

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-MLK3\/RHOE Polyclonal Antibody, Unconjugated (bs-2992R) at 1:200 followed by conjugation to the secondary antibody

Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates probed with MLK3 Polyclonal Antibody, Unconjugated (bs-2992R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLK3 Polyclonal Antibody, Unconjugated (bs-2992R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLK3 Polyclonal Antibody, Unconjugated (bs-2992R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLK3 Polyclonal Antibody, Unconjugated (bs-2992R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MLK3 Polyclonal Antibody, Unconjugated (bs-2992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Cow
Horse

Overview
Catalog #	bs-2992R
Product Name	MLK3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	Due to the similarity of this protein to MLK1 2 and 4, there is a chance that this antibody will cross-react based on homology.
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MLK3
Immunogen Range	355-460/847
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4296
Swiss Prot	Q16584
Subcellular location	Cytoplasm
Synonyms	MLK3; PTK1; SPRK; MLK-3; MEKK11; Mitogen-activated protein kinase kinase kinase 11; Mixed lineage kinase 3; Src-homology 3 domain-containing proline-rich kinase; MAP3K11
Background	Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200