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SPHK2 Polyclonal Antibody

Mouse stomach lysates probed with SPHK2 Polyclonal Antibody, unconjugated (bs-2653R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Mouse stomach lysates probed with SPHK2 Polyclonal Antibody, unconjugated (bs-2653R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF
  • ICC/IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-2653r-100ul
Product Name SPHK2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SPHK2
Immunogen Range 101-200/654
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 56848
Swiss Prot Q9NRA0
Subcellular location Cytoplasm, Cell membrane
Synonyms SK 2; SK-2; SPK 2; SPK-2; Sphingosine kinase 2; SPHK2
Background Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Application Dilution
WB =1:500-2000
ELISA ELISA=1:5000-10000
IHC-P IHC-P=1:100-500
IHC-F IHC-F=1:100-500
IF IF=1:100-500
ICC/IF ICC/IF=1:100

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