IL-22BP/IL22RA2 Polyclonal Antibody

$Mouse splenocytes probed with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

Mouse splenocytes probed with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

$Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n$

Mouse spleen lysates probed with IL22BP Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\\n

$Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Lane 1: Rat testis lysates; probed with IL-22BP\/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat testis lysates; probed with IL-22BP\/IL22RA2 Polyclonal Antibody, unconjugated (bs-2625R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Mouse Spleen Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).$

Mouse Spleen Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with IL-22BP\/IL22RA2 Polyclonal Antibody, Unconjugated (bs-2625R) at 1:200 for 30 min at room temperature. The secondary antibody, Goat anti-rabbit IgG-PE, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse
Rat

Predicted Reactivity

Human

Overview
Catalog #	bs-2625R
Product Name	IL-22BP/IL22RA2 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse, Rat
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human IL22RA2
Immunogen Range	165-263/263
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	116379
Swiss Prot	Q969J5
Subcellular location	Secreted
Synonyms	Interleukin-22 receptor subunit alpha-2; IL-22 receptor subunit alpha-2; IL-22R-alpha-2; IL-22RA2; Cytokine receptor class-II member 10; Cytokine receptor family 2 member 10; CRF2-10; Cytokine receptor family type 2, soluble 1; CRF2-S1; Interleukin-22-binding protein; IL-22BP; IL22BP; ZcytoR16; IL22RA2; UNQ5793/PRO19598/PRO19822
Background	IL22RA2 is a member of the class II cytokine receptor family. The soluble protein specifically binds to and inhibits interleukin 22 activity by blocking the interaction of interleukin 22 with its cell surface receptor. IL22RA2 may be important in the regulation of inflammatory response, and has been implicated in the regulation of tumorigenesis in the colon. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Isoform 2 is a receptor for IL22, and may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Isoform 1 may play a role in establishing and maintaining a successful pregnancy.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200