Glycophorin A Polyclonal Antibody

$FACS Analysis of Glycophorin A and phospho-AMPK alpha 1\/2 (Thr172\/183) in Red Blood Cells in WT and AMPK alpha 1 knockout mice using Rabbit Anti-GPA Polyclonal Antibody (bs-2575R-PE) and Rabbit anti-pAMPK alpha1\/2 Thr172\/183 (bs-4002R-Cy7).\\nImage kindly submitted by Nasrul Hoda, PhD, Georgia Regents University$

FACS Analysis of Glycophorin A and phospho-AMPK alpha 1\/2 (Thr172\/183) in Red Blood Cells in WT and AMPK alpha 1 knockout mice using Rabbit Anti-GPA Polyclonal Antibody (bs-2575R-PE) and Rabbit anti-pAMPK alpha1\/2 Thr172\/183 (bs-4002R-Cy7).\\nImage kindly submitted by Nasrul Hoda, PhD, Georgia Regents University

Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Lung lysates; Lane 3: Mouse Kidney lysates; Lane 4: Mouse Placenta lysates ; Lane 5: Mouse Spleen lysates probed with Glycophorin A Polyclonal Antibody, Unconjugated (bs-2575R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human K562 cell lysates; Lane 2: Human HL60 cell lysates; Lane 3: Human Molt-4 cell lysates; Lane 4: Human Jurkat cell lysates ; Lane 5: Human 293T cell lysates probed with Glycophorin A Polyclonal Antibody, Unconjugated (bs-2575R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Mouse

Overview
Catalog #	bs-2575R
Product Name	Glycophorin A Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse Glycophorin A
Immunogen Range	74-150/150
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2993
Swiss Prot	P14220
Subcellular location	Cell membrane
Synonyms	Glycophorin A; AI853584; CD235a; GPA; Gypa; MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; Glycophorin A MNS blood group; GlycophorinA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HgpMiV; HgpMiX; HgpMiXI; HGpStaC; MN; MN sialoglycoprotein; MNS; PAS-2; PAS2.
Background	Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors and also binds influenza virus.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200