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PTPN11 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (PTPN11) Polyclonal Antibody, Unconjugated (bs-25352R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPN11) Polyclonal Antibody, Unconjugated (bs-25352R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-25352r-100ul
Product Name PTPN11 Polyclonal Antibody
Applications IHC-P, IHC-F, IF, Flow-Cyt
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PTPN11
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5781
Swiss Prot Q06124
Subcellular location Cytoplasm
Synonyms SHP2; PTN11_HUMAN; BPTP 3; BPTP3; CFC; MGC14433; SHP 2; Noonan syndrome 1; Noonan syndrome 1 protein tyrosine phosphatase 2C; NS 1; NS1; Protein tyrosine phosphatase 2C; Protein Tyrosine Phosphatase Non receptor Type 11; PTP 1D; PTP 2C; PTP1D; PTP2C; PTPN 11; SAP2; SH PTP2; SH PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHIP2; SHP 2; SHP-2; SHPTP 2; SHPTP2; SHPTP3; SIT protein precursor; Syp; Tyrosine protein phosphatase non receptor type 11; Src homology 2 (SH2) domain containing phosphotyrosinephosphatase 2.
Application Dilution
IHC-P =1:100-500
IHC-F IHC-F=1:400-800
IF IF=1:100-500
Flow-Cyt Flow-Cyt=1ug/Test

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