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DDX3

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (DDX3 ) Polyclonal Antibody, Unconjugated (bs-25033R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DDX3 ) Polyclonal Antibody, Unconjugated (bs-25033R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Sheep
  • Pig
  • Horse
Overview
Catalog # bs-25033r-100ul
Product Name DDX3
Applications IHC-P, IHC-F, IF
Reactivity Human
Predicted Reactivity Mouse, Rat, Sheep, Pig, Horse
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DDX3
Immunogen Range 1-100/662
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1654
Swiss Prot O00571
Subcellular location Cytoplasm, Nucleus
Synonyms ATP dependent RNA helicase DDX3X; ATP-dependent RNA helicase DDX3X; CAP Rf; DBX; DDX14; DDX3X; DDX3X_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked; DEAD box; DEAD box protein 3; DEAD box protein 3 X-chromosomal; DEAD box X isoform; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3; DEAD/H box 3; Fibroblast Growth Factor Inducible 14; Fin14; Helicase like protein 2; Helicase-like protein 2; HLP2; X isoform; X-chromosomal.
Background ATP-dependent RNA helicase. Acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. Also involved in HIV-1 replication. Interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.
Application Dilution
IHC-P =1:400-800
IHC-F IHC-F=1:400-800
IF IF=1:100-500

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