CD69 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Lane 1: mouse intestine lysates Lane 2: mouse lung lysates probed with Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 22kD. Observed band size: 22kD.$

Lane 1: mouse intestine lysates Lane 2: mouse lung lysates probed with Anti CD69\/CLEC2C\/AIM Polyclonal Antibody, Unconjugated (bs-2499R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 22kD. Observed band size: 22kD.

Lane 1: U937 cell lysates probed with CD69 Polyclonal Antibody, Unconjugated (bs-2499R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Blood cell lysates; Lane 2: Mouse Spleen lysates probed with CD69 Polyclonal Antibody, Unconjugated (bs-2499R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bs-2499R
Product Name	CD69 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD69
Immunogen Range	101-199/199
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	969
Swiss Prot	Q07108
Subcellular location	Cell membrane
Synonyms	AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26; Early activation antigen CD69; Activation inducer molecule; C-type lectin domain family 2 member C; Early T-cell activation antigen p6; Leukocyte surface antigen Leu-23; CD69
Background	Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200