Apelin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody, DAB, and counter-staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Apelin Polyclonal Antibody, Unconjugated (bs-2425R) at 1:400 overnight at 4\u00b0C, followed by secondary antibody, DAB, and counter-staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2425R
Product Name	Apelin Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	The antibody's immunogen sequence is derived from the Apelin-13 peptide. Therefore the Antibody can detect each of the active peptides that can be produced via proteolytic processing of the Apelin precursor.
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Apelin
Immunogen Range	65-77/77
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8862
Swiss Prot	Q9ULZ1
Subcellular location	Secreted
Synonyms	AGTRL1 ligand; APEL; APEL_HUMAN; Apelin-13; Apelin13; Apelin 13; APJ endogenous ligand; Apln; XNPEP2
Background	Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200