Amphiphysin Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Amphiphysin Polyclonal Antibody, Unconjugated (bs-2409R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Amphiphysin Polyclonal Antibody, Unconjugated (bs-2409R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2409R
Product Name	Amphiphysin Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Amphiphysin
Immunogen Range	101-200/695
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	273
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Amphiphysin; Amp; AMPH 1; AMPH1; Amphiphysin Stf-Man syndrome with breast cancer 128kD autoantigen; Amphiphysin Stf-Man syndrome with breast cancer 128kDa autoantigen; Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stf man syndrome with breast cancer 128kD autoantigen; Stf man syndrome with breast cancer 128kDa autoantigen; AMPH_HUMAN.
Background	Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for auto against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200