ST2 Polyclonal Antibody

$Huvec (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ST2 Antibody (bs-2382R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n$

Huvec (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ST2 Antibody (bs-2382R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-2382R
Product Name	ST2 Polyclonal Antibody
Applications	FCM
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Interleukin 1 receptor like 1
Immunogen Range	11-110/556
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9173
Swiss Prot	Q01638
Subcellular location	Secreted, Cell membrane
Synonyms	T1; ST2; DER4; ST2L; ST2V; FIT-1; IL33R; Interleukin-1 receptor-like 1; Protein ST2; IL1RL1
Background	Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.
Application Dilution
FCM	1:20-100