phospho-Nrf2 (Ser40) Polyclonal Antibody

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Mouse BV-2 cell lysates probed with phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-23531R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Mouse BV-2 cell lysates probed with phospho-Nrf2 (Ser40) Polyclonal Antibody, Unconjugated (bs-23531R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Chicken

Overview
Catalog #	bs-23531R
Product Name	phospho-Nrf2 (Ser40) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF, ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human phospho-Nrf2 (Ser40)
Modification Site	Ser40
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4780
Swiss Prot	Q16236
Subcellular location	Cytoplasm, Nucleus
Synonyms	Nrf2 (phospho S40); p-Nrf2 (phospho S40); Nuclear factor-E2 related factor2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN.
Background	Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC	1:100-500