CCR2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCR2 Polyclonal Antibody (bs-23026R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CCR2 Polyclonal Antibody (bs-23026R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Raw264.7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CCR-2 Polyclonal Antibody(bs-23026R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Lane 1: Mouse Spleen lysates; Lane 2: Mouse Lymph node lysates; Lane 3: Mouse Heart lysates; Lane 4: Rat Spleen lysates probed with CCR2 Polyclonal Antibody, Unconjugated (bs-23026R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
FCM
IHC-P
IHC-F
ICC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-23026R
Product Name	CCR2 Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, ICC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse CCR2
Immunogen Range	301-373/373
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	12772
Swiss Prot	P51683
Subcellular location	Cell membrane
Synonyms	C-C chemokine receptor type 2; JE/FIC receptor; MCP-1 receptor; CD192; Cmkbr2
Background	Receptor for the CCL2, CCL7 and CCL12 chemokines. Transduces a signal by increasing intracellular calcium ion levels.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
ICC	1:100-500