CD162 Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Anti-CD162 Polyclonal Antibody, Unconjugated (bs-2301R) followed by conjugation to the secondary antibody

$U937 cells probed with \\tAnti-CD162 Polyclonal Antibody, Unconjugated (bs-2301R) at 0.2ug for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).$

U937 cells probed with \\tAnti-CD162 Polyclonal Antibody, Unconjugated (bs-2301R) at 0.2ug for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Lane 1: Jurkat cell lysates; Lane 2: MOLT-4 cell lysates probed with CD162 Polyclonal Antibody, Unconjugated (bs-2301R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Raji cell lysates; Lane 2: U937 cell lysates probed with CD162 Polyclonal Antibody, Unconjugated (bs-2301R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bs-2301R
Product Name	CD162 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PSGL-1/CD162
Immunogen Range	251-350/412
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6404
Swiss Prot	Q14242
Subcellular location	Cell membrane
Synonyms	CLA; CD162; PSGL1; PSGL-1; P-selectin glycoprotein ligand 1; Selectin P ligand; SELPLG
Background	A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400