CD80 Polyclonal Antibody

$Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-B7-1\/CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-B7-1\/CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse spleen lysates probed with CD80 Polyclonal Antibody, Unconjugated (bs-2211R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-2211R
Product Name	CD80 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat CD80
Immunogen Range	251-321/321
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	25408
Swiss Prot	G3V671
Subcellular location	Cell membrane
Synonyms	B7-1; Activation B7 1 antigen; Activation B71 antigen; B lymphocyte activation antigen B7; B7; BB1; CD 80; CD28 antigen ligand 1 B7 1 antigen; CD28 antigen ligand 1 B71 antigen; CD28LG; CD28LG1; CD80 antigen; CD80 antigen precursor;CD80 molecule; Costimulatory factor CD80; CTLA 4 counter receptor B7.1; CTLA-4 counter-receptor B7.1; LAB 7; LAB7; T lymphocyte activation antigen CD80; CD80_MOUSE.
Background	CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500