Uromodulin Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-MCKD2\/UMOD Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-MCKD2\/UMOD Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Lane 1: Rat Kidney lysates; Lane 2: Human Kidney Carcinoma lysates; Probed with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:300, overnight at 4˚C. Followed by secondary antibody (bs-0295G-HRP) at 1:3000 for 90min at 37˚C.

Mouse kidney lysates probed with Rabbit Anti-Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$Mouse kidney cells were fixed with 2% paraformaldehyde for 10 min at 37\u2103. Blocking was performed with 1 X PBS containing 0.5% BSA + 10% goat serum for 1 hour. Uromodulin Polyclonal Antibody (bs-2189R, 1\u03bcg\/1x10^6 cells) was incubated with the cells for 30 min at room temperature. Then a Goat Anti-rabbit IgG\/FITC (bs-0295G-FITC) secondary antibody was added at 1:200 for 40 mins at room temperature. The graph compares primary antibody (green) to unstained cells (dark blue), secondary only (light blue) and isotype control (orange; bs-0295P).$

Mouse kidney cells were fixed with 2% paraformaldehyde for 10 min at 37\u2103. Blocking was performed with 1 X PBS containing 0.5% BSA + 10% goat serum for 1 hour. Uromodulin Polyclonal Antibody (bs-2189R, 1\u03bcg\/1x10^6 cells) was incubated with the cells for 30 min at room temperature. Then a Goat Anti-rabbit IgG\/FITC (bs-0295G-FITC) secondary antibody was added at 1:200 for 40 mins at room temperature. The graph compares primary antibody (green) to unstained cells (dark blue), secondary only (light blue) and isotype control (orange; bs-0295P).

$Paraformaldehyde-fixed, paraffin-embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, FITC Conjugated secondary antibody (bs-0295G-FITC) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.$

Paraformaldehyde-fixed, paraffin-embedded Human Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, FITC Conjugated secondary antibody (bs-0295G-FITC) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.

$Paraformaldehyde-fixed, paraffin-embedded Rat Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, Cy3 Conjugated secondary antibody (bs-0295G-Cy3) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.$

Paraformaldehyde-fixed, paraffin-embedded Rat Kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Uromodulin Polyclonal Antibody, Unconjugated (bs-2189R) at 1:200 overnight at 4\u00b0C. A Goat Anti-Rabbit IgG, Cy3 Conjugated secondary antibody (bs-0295G-Cy3) was used for 40 mins at 37\u00b0C. DAPI (5ug\/ml, blue) was used for nuclei staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-2189R
Product Name	Uromodulin Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Human Uromodulin
Immunogen Range	351-450/642
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7369
Swiss Prot	P07911
Subcellular location	Secreted, Cell membrane
Synonyms	ADMCKD2; FJHN; HNFJ; HNFJ1; MCKD2; medullary cystic kidney disease 2 autosomal dominant; Tamm Horsfall glycoprotein; Tamm Horsfall urinary glycoprotein; Tamm-Horsfall urinary glycoprotein; THGP; THP; Umod; UROM_HUMAN; Uromodulin.
Background	Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelia. Uromodulin, secreted form: Secreted into urine after proteolytical cleavage. Into the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and modulates formation of supersaturated salts and their crystals.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200