GSK3 Alpha/Beta(Tyr279+Tyr216) Antibody

$Mouse heart lysates probed with Rabbit Anti-GSK3 alpha\/beta (Tyr279+Tyr216) Polyclonal Antibody, Unconjugated (bs-2073R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.$

Mouse heart lysates probed with Rabbit Anti-GSK3 alpha\/beta (Tyr279+Tyr216) Polyclonal Antibody, Unconjugated (bs-2073R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

$RSC96 cells probed with Rabbit Anti-GSK3 Alpha\/Beta(Tyr279+Tyr216) Polyclonal Antibody (bs-2073R), 3ug,for 20 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:200 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n$

RSC96 cells probed with Rabbit Anti-GSK3 Alpha\/Beta(Tyr279+Tyr216) Polyclonal Antibody (bs-2073R), 3ug,for 20 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:200 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n

$Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Pancreas lysates; Lane 5: Mouse Thymus lysates; Lane 6: Rat Muscle lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Cerebrum lysates; Lane 9: Rat Pancreas lysates; Lane 10: Rat Thymus lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human A431 cell lysates; Lane 13: Human U-2OS cell lysates probed with GSK3 Alpha\/Beta Polyclonal Antibody, Unconjugated (bs-2073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Muscle lysates; Lane 2: Mouse Liver lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Mouse Pancreas lysates; Lane 5: Mouse Thymus lysates; Lane 6: Rat Muscle lysates; Lane 7: Rat Liver lysates; Lane 8: Rat Cerebrum lysates; Lane 9: Rat Pancreas lysates; Lane 10: Rat Thymus lysates; Lane 11: Human MCF-7 cell lysates; Lane 12: Human A431 cell lysates; Lane 13: Human U-2OS cell lysates probed with GSK3 Alpha\/Beta Polyclonal Antibody, Unconjugated (bs-2073R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Chicken
Rabbit
Guinea Pig

Overview
Catalog #	bs-2073R
Product Name	GSK3 Alpha/Beta(Tyr279+Tyr216) Antibody
Applications	WB, ELISA, FCM, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Guinea Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human GSK3 Alpha/Beta around the phosphorylation site of Tyr279/Tyr216
Modification Site	Tyr279+Tyr216
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2931
Swiss Prot	P49840
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A
Background	Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200