CD43 Polyclonal Antibody

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Rat Spleen tissue lysates probed with CD43 Polyclonal Antibody, Unconjugated (bs-20324R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Thymus tissue lysates; Lane 3: Rat Spleen tissue lysates probed with CD43 Polyclonal Antibody, Unconjugated (bs-20324R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Mouse

Predicted Reactivity

Human
Rat

Overview
Catalog #	bs-20324R
Product Name	CD43 Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CD43
Immunogen Range	101-200/400
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6693
Swiss Prot	P16150
Subcellular location	Cell membrane
Synonyms	LSN; CD43; GALGP; GPL115; Leukosialin; Galactoglycoprotein; Leukocyte sialoglycoprotein; Sialophorin; SPN
Background	One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000