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NIT2 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat brain labeled with Anti- NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti- NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo tissue labeled Anti-NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse embryo tissue labeled Anti-NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
A431 cell lysates probed with NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A431 cell lysates probed with NIT2 Polyclonal Antibody, Unconjugated (bs-2012R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-2012R
Product Name NIT2 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat NIT2
Immunogen Range 41-140/267
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 288174
Swiss Prot Q497B0
Subcellular location Cytoplasm
Synonyms RGD1310494; Omega-amidase NIT2; Nitrilase homolog 2; Nit2
Background Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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