LAMP-1 Polyclonal Antibody

Formalin-fixed and paraffin embedded rat kidney labeled with Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Mouse lung lysates probed with Rabbit Anti-LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

RSC96 probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Image provided by One World Lab validation initiative. PC3 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at [1:100] in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Human U937 cells probed with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

$Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LAMP-1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:400 overnight at 4\u00b0C, DAB staining.

$A549 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated(bs-1970R) at 1:100 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.$

A549 cells were stained with LAMP-1 Polyclonal Antibody, Unconjugated(bs-1970R) at 1:100 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

$Lane 1: Human HT-1080 cell lysates; Lane 2: Human JAR cell lysates probed with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HT-1080 cell lysates; Lane 2: Human JAR cell lysates probed with LAMP1 Polyclonal Antibody, Unconjugated (bs-1970R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat
Monkey

Overview
Catalog #	bs-1970R
Product Name	LAMP-1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat, Monkey
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human LAMP-1
Immunogen Range	301-417/417
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3916
Swiss Prot	P11279
Subcellular location	Cytoplasm, Cell membrane
Synonyms	LAMPA; CD17a; LGP12; Lysosome-associated membrane glycoprotein 1; LAMP-1; Lysosome-associated membrane protein 1; CD17 antigen-like family member A; LAMP1
Background	Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500