UCP-2 Polyclonal Antibody

Formalin-fixed and paraffin embedded human endometrium labeled with Anti-UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 followed by conjugation to the secondary antibody and DAB staining

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:400 overnight at 4\u00b0C, DAB staining.

$Human Hela Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:100 for 30 min at room temperature. The secondary antibody, F(ab\u2019)2 fragment Goat anti-rabbit IgG-FITC, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).$

Human Hela Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Cells were stained with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:100 for 30 min at room temperature. The secondary antibody, F(ab\u2019)2 fragment Goat anti-rabbit IgG-FITC, was used for 40 min at room temperature. Primary Antibody staining (green) is compared to unstained cells (dark blue), secondary only (light blue) and isotype control (orange).

Lane 1: Raw264.7 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: BV-2 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Rat Stomach lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: Siha cell lysates; Lane 4: Du145 cell lysates ; Lane 5: Raw264.7 cell lysates ; Lane 6: Mouse Liver lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Rat Stomach lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: Siha cell lysates; Lane 4: Du145 cell lysates ; Lane 5: Raw264.7 cell lysates ; Lane 6: Mouse Liver lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Stomach lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human A673 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Stomach lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human DU145 cell lysates; Lane 4: Human A673 cell lysates probed with UCP-2 Polyclonal Antibody, Unconjugated (bs-1926R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (UCP-2) polyclonal Antibody, Unconjugated (bs-1926R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Pig
Horse
Rabbit

Overview
Catalog #	bs-1926R
Product Name	UCP-2 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from Mouse UCP-2 (Mitochondrial intermembrane space domain)
Immunogen Range	201-309/309
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	22228
Swiss Prot	P70406
Subcellular location	Cytoplasm, Nucleus
Synonyms	Slc25a8; Mitochondrial uncoupling protein 2; UCP 2; Solute carrier family 25 member 8; UCPH; Ucp2
Background	UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200