PDL2/B7-DC Polyclonal Antibody

$Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-B7-DC\/CD273\/PDL2, Unconjugated (bs-1868R) followed by conjugation to the secondary antibody$

Formalin-fixed and paraffin embedded human cervical cancer labeled with Anti-B7-DC\/CD273\/PDL2, Unconjugated (bs-1868R) followed by conjugation to the secondary antibody

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (bs-1868R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (PDCD1LG2) polyclonal Antibody, Unconjugated (bs-1868R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

$HepG2 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PDCD1LG2 Polyclonal Antibody(bs-1868R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).$

HepG2 cells were\u00a0 incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with PDCD1LG2 Polyclonal Antibody(bs-1868R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Applications

ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Cow

Overview
Catalog #	bs-1868R
Product Name	PDL2/B7-DC Polyclonal Antibody
Applications	ELISA, IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human B7-DC
Immunogen Range	41-150/273
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	80380
Swiss Prot	Q9BQ51
Subcellular location	Secreted, Cell membrane
Synonyms	B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PDCD1LG2
Background	Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity).
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400