MAP1A Polyclonal Antibody

Formalin-fixed and human glioma tissue labeled with Anti-MAP1A Polyclonal Antibody (bs-1847R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded human cervical cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with MAP1A Polyclonal Antibody, Unconjugated (bs-1847R ) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

U87MG cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAP1A Polyclonal Antibody(bs-1847R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).