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Wnt1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse brain lysates probed with Rabbit Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse brain lysates probed with Rabbit Anti-Wnt1 Polyclonal Antibody, Unconjugated (bs-1739R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Horse
  • Chicken
Overview
Catalog # bs-1739R
Product Name Wnt1 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Horse, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Wnt1
Immunogen Range 251-350/370
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7471
Swiss Prot P04628
Subcellular location Secreted
Synonyms INT1; OI15; BMND16; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1
Background Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Has a role in osteoblast function and bone development.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

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