MASP Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti- MASP Polyclonal Antibody, Unconjugated (bs-1723R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

Rat brain lysates probed with Rabbit Anti-MASP Polyclonal Antibody (bs-1723R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MASP1\/MASP3 Polyclonal Antibody, Unconjugated (bs-1723R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MASP1\/MASP3 Polyclonal Antibody, Unconjugated (bs-1723R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

$Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MASP1\/MASP3 Polyclonal Antibody, Unconjugated (bs-1723R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MASP1\/MASP3 Polyclonal Antibody, Unconjugated (bs-1723R) at 1:100 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Applications

WB
ELISA
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-1723R
Product Name	MASP Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human MASP
Immunogen Range	20-120/699
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5648
Swiss Prot	P48740
Subcellular location	Cytoplasm, Cell membrane
Synonyms	3MC1; MAP1; MASP; RaRF; CRARF; MASP3; MAp44; PRSS5; CRARF1; Mannan-binding lectin serine protease 1; Complement factor MASP-3; Complement-activating component of Ra-reactive factor; Mannose-binding lectin-associated serine protease 1; MASP-1; Mannose-binding protein-associated serine protease; Ra-reactive factor serine protease p100; Serine protease 5; MASP1
Background	Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(IHC-P)	1:50-200