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F-Actin Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Chicken

Predicted Reactivity

  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-1571R
Product Name F-Actin Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IF(IHC-P), IF(ICC)
Reactivity Human, Mouse, Rat, Dog, Chicken
Predicted Reactivity Cow, Sheep, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human F-actin capping protein alpha subunit
Immunogen Range 1-50/374
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 60
Swiss Prot P52907
Subcellular location Cytoplasm
Synonyms CapZ alpha-1; F-actin-capping protein subunit alpha-1; CAZA1_HUMAN
Background Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.

Application Dilution
WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200