CCL26 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CCL26 Polyclonal Antibody (bs-15513R) at 1:400 overnight at 4\u00b0C, followed by a SP kit and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with CCL26 Polyclonal Antibody (bs-15513R) at 1:400 overnight at 4\u00b0C, followed by a SP kit and DAB staining.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-15513R
Product Name	CCL26 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat CCL26
Immunogen Range	21-94/94
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	685958
Swiss Prot	D3ZQG2
Subcellular location	Secreted
Synonyms	C-C mot chemokine 26; CCL26; Chemokine C C mot ligand 26; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MIP 4a; MIP 4alpha; SCYA 26; SCYA26; Small inducible cytokine A26; Small inducible cytokine subfamily A Cys member 26; Small inducible cytokine subfamily A member 26; Thymic stroma chemokine 1; TSC1.
Background	Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500