CRAT/CAT1 Polyclonal Antibody

$Lane 1: Mouse muscle lysates; Lane 2: mouse heart lysates probed with CRAT\/CAT1 Polyclonal Antibody, Unconjugated (bs-15397R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.$

Lane 1: Mouse muscle lysates; Lane 2: mouse heart lysates probed with CRAT\/CAT1 Polyclonal Antibody, Unconjugated (bs-15397R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Chicken

Overview
Catalog #	bs-15397R
Product Name	CRAT/CAT1 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CRAT/CAT1
Immunogen Range	301-400/626
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1384
Swiss Prot	P43155
Subcellular location	Cell membrane
Synonyms	CACP_HUMAN; Carnitine acetylase; Carnitine acetyltransferase; Carnitine O acetyltransferase; Carnitine O-acetyltransferase; CAT; CAT1; CrAT;
Background	CRAT is a key enzyme in the metabolic pathway in mitochondria, peroxisomes and endoplasmic reticulum. CRAT catalyzes the reversible transfer of acyl groups from an acyl-CoA thioester to carnitine and regulates the ratio of acylCoA/CoA in the subcellular compartments. Two transcript variants encoding different isoforms have been found for this gene.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500