LIFR/CD118 Polyclonal Antibody

Formalin-fixed and paraffin-embedded human colon carcinoma labeled with Rabbit Anti-CD118 Polyclonal Antibody (bs-1458R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Human U937 cells probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).$

Human U937 cells probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

$MCF-7 lysates probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C$

MCF-7 lysates probed with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

$Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LIFR\/CD118 Polyclonal Antibody, Unconjugated (bs-1458R) at 1:200 overnight at 4\u00b0C, DAB staining.

$Lane 1: Human U-87 MG cell Lysates; Lane 2: Human U2OS cell Lysates; Lane 3: Human HUVEC cell Lysates; Lane 4: Human SH-SY5Y cell Lysates. Probed with LIFR polyclonal Antibody, unconjugated (bs-1458R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U-87 MG cell Lysates; Lane 2: Human U2OS cell Lysates; Lane 3: Human HUVEC cell Lysates; Lane 4: Human SH-SY5Y cell Lysates. Probed with LIFR polyclonal Antibody, unconjugated (bs-1458R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Others

Predicted Reactivity

Mouse
Rat
Dog
Cow

Overview
Catalog #	bs-1458R
Product Name	LIFR/CD118 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Others
Predicted Reactivity	Mouse, Rat, Dog, Cow
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from rat LIFR
Immunogen Range	451-580/1093
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3977
Swiss Prot	O70535
Subcellular location	Extracellular, Cell membrane
Synonyms	SWS; SJS2; STWS; CD118; LIF-R; Leukemia inhibitory factor receptor; LIF receptor; LIFR
Background	Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200