SRF Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-SRF\/SAP2\/MCM1 Polyclonal Antibody, Unconjugated (bs-1401R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-SRF\/SAP2\/MCM1 Polyclonal Antibody, Unconjugated (bs-1401R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Mouse large intestine lysates probed with Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse large intestine lysates probed with Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Mouse muscle lysates probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse muscle lysates probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

$Mouse NIH\/3T3 cells probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.$

Mouse NIH\/3T3 cells probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: 293T cell lysates probed with SRF Polyclonal Antibody, Unconjugated (bs-1401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Overview
Catalog #	bs-1401R
Product Name	SRF Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SRF
Immunogen Range	101-250/508
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6722
Swiss Prot	P11831
Subcellular location	Nucleus
Synonyms	MCM1; Serum response factor; SRF
Background	SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200