Cyclin G Polyclonal Antibody

Formalin-fixed and paraffin embedded human endometrium carcinoma tissue labeled with Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:200 followed by conjugation to the secondary antibody and DAB stainin

Mouse liver lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.

Mouse kidney lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.

Mouse spleen lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.

$HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (bs-1389R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (bs-1389R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-1389R
Product Name	Cyclin G Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Cyclin G
Immunogen Range	181-295/295
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	900
Swiss Prot	P51959
Subcellular location	Nucleus
Synonyms	CCNG; Cyclin-G1; Cyclin-G; CCNG1; CYCG1
Background	May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200