GPATCH3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-GPATCH3 Polyclonal Antibody, Unconjugated (bs-13500R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining$

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-GPATCH3 Polyclonal Antibody, Unconjugated (bs-13500R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Cow
Pig
Horse

Overview
Catalog #	bs-13500R
Product Name	GPATCH3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GPATCH3
Immunogen Range	331-430/525
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm, Nucleus
Synonyms	DKFZp686E0221; FLJ12455; G patch domain containing 3; G patch domain containing protein 3; G patch domain-containing protein 3; GPATC3; GPATCH3; GPTC3_HUMAN.
Background	GPATCH3
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500