Gametogenetin Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-GGN\/Gametogenetin Polyclonal Antibody, Unconjugated (bs-13347R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat testis labeled with Rabbit Anti-GGN\/Gametogenetin Polyclonal Antibody, Unconjugated (bs-13347R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$A549 Cell lysates probed with Gametogenetin Polyclonal Antibody, unconjugated (bs-13347R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

A549 Cell lysates probed with Gametogenetin Polyclonal Antibody, unconjugated (bs-13347R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Pig

Overview
Catalog #	bs-13347R
Product Name	Gametogenetin Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human GGN/Gametogenetin
Immunogen Range	5-100/652
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	199720
Subcellular location	Cytoplasm, Nucleus
Synonyms	FLJ35713; Gametogenetin; MGC33369; GGN_HUMAN; GGN
Background	Gametogenetin is a 652 amino acid protein that is primarily expressed in testis and ovary. Interacting with POG (proliferation of germ cells), GGNBP1 and LCRG1, gametogenetin is likely involved in spermatogenesis. Gametogenetin exists as three alternatively spliced isoforms commonly known as gametogenetin protein 1 (GGN1), gametogenetin protein 2 (GGN2) and gametogenetin protein 3 (GGN3), which localize to nuclear membrane, cytoplasm and nucleus/nucleoli, respectively. GGN1 contains two transmembrane domains in its N-terminal half and 2 C-terminal arginine- and lysine-rich nucleolar targeting sequences. GGN1 and GGN3 are likely linked to germ cell development and both GGN1 and GGN2 may be involved in cell trafficking.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500