VEGFA Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rabbit liver tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:600 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rabbit kidney tissue labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (bs-1313R) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rabbit meniscuslabeled with Anti-VEGF Polyclonal Antibody, Unconjugated (bs-1313R) followed by conjugation to the secondary antibody and DAPI staining

Rat lung lysates probed with Anti VEGF Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 24kD. Observed band size: 24kD.

$Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.$

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.

$Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.\r\n$

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.\r\n

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat
Rabbit

Predicted Reactivity

Dog
Cow
Chicken
Pig

Overview
Catalog #	bs-1313R
Product Name	VEGFA Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat, Rabbit
Predicted Reactivity	Dog, Cow, Chicken, Pig
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human VEGF
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7422
Swiss Prot	P15692
Subcellular location	Secreted, Extracellular matrix
Synonyms	Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; VEGF-A; Vascular permeability factor (VPF);
Background	This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. Function : Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Application Dilution
WB
IHC-P	1:200-400
IF(IHC-P)	1:50-200