Smac/DIABLO Polyclonal Antibody

$Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Smac\/DIABLO Polyclonal Antibody, Unconjugated (bs-1298R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Smac\/DIABLO Polyclonal Antibody, Unconjugated (bs-1298R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Gastric Cancer Cells labeled with Anti-Smac\/DIABLO Polyclonal Antibody, Unconjugated (bs-1298R) followed by conjugation to the secondary antibody$

Gastric Cancer Cells labeled with Anti-Smac\/DIABLO Polyclonal Antibody, Unconjugated (bs-1298R) followed by conjugation to the secondary antibody

Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated (bs-10014R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD

Applications

WB
ELISA
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Pig
Horse
Chicken

Overview
Catalog #	bs-1298R
Product Name	Smac/DIABLO Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Smac
Immunogen Range	131-239/239
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	56616
Swiss Prot	Q9NR28
Subcellular location	Cytoplasm
Synonyms	SMAC; DFNA64; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; DIABLO
Background	Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IF(ICC)	1:50-200